Genome-wide surveillance of genital HSV-1 from multiple anatomic sites over time.
Shipley MM, Renner DW, Ott M, Bloom DC, Koelle DM, Johnston C, Szpara ML – Journal of Infectious Diseases [in press]
Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons.
Mangold CA, Yao PJ, Du M, Freeman WM, Benkovic SJ, Szpara ML – Journal of Neurochemistry [DOI: 10.1111/jnc.14304] [PDF]
Impacts of genome-wide analyses on our understanding of human herpesvirus diversity and evolution.
Renner DW and Szpara ML – Journal of Virology [DOI: 10.1128/JVI.00908-17] [PDF]
Inferred father-to-son transmission of herpes simplex virus results in near-perfect preservation of viral genome identity and in vivo phenotypes.
Pandey U, Renner DW, Thompson RL, Szpara ML, Sawtell NM – Scientific Reports [PDF]
Differentiated SH-SY5Y human cells provide a reductionist model of HSV-1 neurotropism
Shipley MM, Mangold CA, Kuny CV, Szpara ML – Journal of Virology [PDF]
Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
Aravantinou M, Mizenina O, Calenda G, Kenney J, Frank I, Lifson JD, Szpara M, Jing L, Koelle DM, Teleshova N, Grasperge B, Blanchard J, Gettie A, Martinelli E, Derby N – Frontiers in Microbiology [PDF]
A phylogenomic analysis of Marek’s disease virus reveals independent paths to virulence in Eurasia and North America
Trimpert J, Groenke N, Jenckel M, He S, Kunec D, Szpara ML, Spatz SJ, Osterrieder N, McMahon DP – Evolutionary Applications [PDF]
A model of genital herpes simplex virus Type 1 infection in Rhesus Macaques
Aravantinou M, Frank I, Arrode-Bruses G, Szpara M, Grasperge B, Blanchard J, Gettie A, Derby N, Martinelli E – Journal of Medical Primatology [PDF]
DNA from dust: comparative genomics of large DNA viruses in field surveillance samples
Pandey U, Bell AS, Renner D, Kennedy DA, Shreve J, Cairns CL, Jones MJ, Dunn PA, Read AF, Szpara ML – mSphere [PDF]
- Public repository for data from this paper: https://scholarsphere.psu.edu/collections/1544bp14j
Viral forensic genomics reveals the relatedness of classic herpes simplex virus strains KOS, KOS63, and KOS79
Bowen CD, Renner DW, Shreve JT, Tafuri Y, PayneKM, Dix RD, Kinchington PR, Gatherer D, Szpara ML – Virology (Elsevier) [PDF]
Differentiation of the SH-SY5Y human neuroblastoma cell line
Shipley MM, Mangold CA, Szpara ML – Journal of Visualized Experiments [PDF] [Movie]
VirAmp: A Galaxy-based viral genome assembly pipeline
Wan Y, Renner DW, Albert I, Szpara ML – GigaScience [PDF]
Rapid genome assembly and comparison decodes intra-strain variation in human alpha-herpesviruses
Parsons LR, Tafuri YR, Shreve JT, Bowen CD, Shipley MM, Enquist LW, Szpara ML – mBio [PDF]
- How-to documentation for VirGA: http://virga.readthedocs.org/en/latest/
- Code repository for VirGA: https://bitbucket.org/szparalab
- A freely-accessible Virtual Machine (VM) that can run VirGA: https://www.dropbox.com/sh/szsnelelpbs4qip/AAAohfvHWBGG_HMLL6pJgmTca?dl=0
- Public repository for data used in this paper: https://scholarsphere.psu.edu/collections/sf268c193
Genome sequence of the anterograde-spread defective herpes simplex virus 1 strain MacIntyre
Szpara ML, Tafuri YR, Parsons L, Shreve JT, Engel EA, Enquist LW – Genome Announcements [PDF]
Isolation of Herpes Simplex Virus Nucleocapsid DNA
ML Szpara – Methods in Molecular Biology [PDF]
Evolution and diversity in human herpes simplex virus genomes
ML Szpara, D Gatherer, A Ochoa, B Greenbaum, A Dolan, RJ Bowden, LW Enquist, M Legendre, AJ Davison – Journal of Virology [PDF]
- Data website: http://szparalab.psu.edu/hsv-diversity/
Herpes simplex virus 1 pUL34 plays a critical role in cell-to-cell spread of virus in addition to its role in virus replication
AC Haugo, ML Szpara, L Parsons, LW Enquist, RJ Roller – Journal of Virology [PDF]
A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses
ML Szpara, YR Tafuri, L Parsons, SR Shamim, KJ Verstrepen, M Legendre, LW Enquist – PLoS Pathogens [PDF]
- Data website: http://viro-genome.princeton.edu/PRV
- How-to documentation: http://genomics-pubs.princeton.edu/prv/
Sequence variability in clinical and laboratory isolates of herpes simplex virus 1 reveals new mutations
ML Szpara, L Parsons, LW Enquist – Journal of Virology [PDF] [Data website]
- Data website: http://viro-genome.princeton.edu/HSV
A common neuronal response to alphaherpesvirus infection
ML Szpara, O Kobiler, LW Enquist – Journal of Neuroimmune Pharmacology [PDF]
Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus
AE Granstedt, ML Szpara, B Kuhn, SS Wang, LW Enquist – PLoS One [PDF]
Compartmented neuron cultures for directional infection by alpha herpesviruses
D Curanović, TH Ch’ng, M Szpara, L Enquist – Current Protocols in Cell Biology [PDF]
Analysis of gene expression during neurite outgrowth and regeneration
ML Szpara, K Vranizan, YC Tai, CS Goodman, TP Speed, J Ngai – BMC Neuroscience [PDF]
Regulation of pontine neurite morphology by target-derived signals
SK Hansen, ML Szpara, TA Serafini – Molecular Brain Research [PDF]
- Moriah will be giving a talk in the HSV Satellite of the 43rd Annual International Herpesvirus Workshop in Vancouver, Canada. Chad Kuny, Mackenzie Shipley, and Moriah will be presenting a total of four posters at the Workshop. We are looking forward to seeing great science & many collaborators there!
– Colleen Mangold, Chris Bowen, and our collaborator-postdoc Lisa Akhtar will be giving talks at the American Society for Virology (ASV) Annual meeting in Maryland. Molly Rathbun will be presenting a poster, and Moriah will be there to soak up the science & cheer everyone on!
- Colleen Mangold will be giving a talk at the University of Pennsylvania Herpesvirus: Pathogenesis & Cancer Symposium on June 1. The whole lab takes a road trip to attend the day-long U. Penn symposium every year.
-Moriah will be giving a talk at the Spear Colloquium in honor of Dr. Patricia Spear, at Northwestern University’s Feinberg School of Medicine, Department of Microbiology-Immunology in Chicago, IL. It will be an honor to meet Patricia, one of the founding members of our field!
– Mackenzie Shipley will be giving a talk & a poster, and Utsav Pandey will be giving a poster at the American Society for Microbiology (ASM) Microbe 2018 meeting in Atlanta, GA. This will be Utsav’s final presentation (that we know of!) for the lab. Kudos to both of our senior graduate students for their excellent research!
– Moriah will give an invited talk & Daniel Renner a poster at the 2018 Virus Genomics and Evolution meeting in Cambridge (UK). The meeting is at the Wellcome Genome Campus, heart of an amazing amount of progress in sequencing, genomics, and computational biology. We are excited to be there!
– Colleen Mangold will be giving at talk at the Center for Molecular Investigation of Neurological Disorders (CMIND) Annual Retreat here at University Park.
- Utsav Pandey received his PhD at the Spring Commencement of Penn State University — congratulations on your new degree, Utsav!
– Moriah gave a talk & Mackenzie Shipley gave a poster at the 2018 Colorado Alphaherpesvirus Latency Symposium (CALS) in Vail, CO. It was Mackenzie’s first meeting at CALS, and she agrees that the small format provides a great opportunity to talk with colleagues and share new science. Thank you to Randy Cohrs, Nick Baird, & Maria Nagel for organizing this!
- Mackenzie Shipley gave a Center for Infectious Disease Dynamics (CIDD) Graduate Student Seminar here at PSU. Each student or postdoc has an opportunity to do this once during their training here. Mackenzie did a great job!
– Molly Rathbun gave a poster at the 2018 Penn State Institute for CyberScience (ICS) Symposium. Thanks Molly!
- Utsav Pandey successfully defended his PhD thesis — first graduate of the Szpara lab! He will be accepting a Clinical Diagnostic Microbiology Fellowship in California this July. Read more about these here, at the ASM Committee on Postgraduate Education Programs (CPEP). Congratulations on all of your achievements, Utsav!!!
– Molly Rathbun gave her first-ever presentation for Virology@PSU, and presented her ideas for her future thesis. Good practice for her comprehensive examination in the year ahead!
– Moriah gave a talk about our new neonatal HSV research at the Viruses 2018—Breakthroughs in Viral Replication meeting in Barcelona, Spain. You can see some photos at the MDPI Viruses blog post here. It’s was an excellent meeting!
- Colleen Mangold gave a talk in December for the Molecular Virology super-group, which has grown to include the labs of Craig Cameron, Susan Hafenstein, Joyce Jose, Suresh Kuchipudi, Tony Schmitt, Troy Sutton, & our lab.
– Molly Rathbun passed her candidacy exam — the first of several landmarks on the path to a PhD. Congrats Molly!
– Our fourth annual lab retreat at Raystown Lake was a success — lots of science & news to reflect on, and more to look forward to in the year ahead!
- A new paper with Utsav Pandey, Daniel Renner, Rick Thompson (University of Cinncinnati), and Nancy Sawtell (Cincinnati Children’s Hospital) is accepted & published at Scientific Reports. Key highlight: This is the first-ever view of HSV-1 transmission in a family setting to include both genomic & in vivo phenotypic analyses. Congratulations Utsav and all for this accomplishment!
- A new paper by Mackenzie Shipley, Colleen Mangold, and Chad Kuny is accepted & online at JVI. Key highlight: HSV-1 infection proceeds differently in terminally-differentiated neuronal cells, than in their undifferentiated precursors. Congratulations Mackenzie, Colleen, & Chad!
- Moriah Szpara gave the Priscilla Schaffer Memorial Lecture at the International Herpesvirus Workshop (IHW) in Ghent, Belgium. Thanks to Herman Favoreel & Hans Nauwynck for organizing a great meeting! Moriah also organized a meeting on genomics methods, prior to the main meeting. Thank you to all the groups that participated in a very fruitful discussion.
– Christian Thomas from Dillard University completed a summer research project in the lab, working with mentorship from Utsav Pandey. His visit was sponsored by Penn State’s Summer Research Opportunities Program (SROP). Christian did a great job with his final presenation!
- Chad Kuny gave his first talk from new research in the Szpara lab, at the University of Pennsylvania’s 17th Annual Herpesvirus: Pathogenesis and Cancer Symposium. Way to go, Chad!
– The lab was represented at the ASM Microbe meeting in New Orleans, with posters presented by Utsav Pandey (grad student) & Kokila Shankar (senior undergraduate). Kokila participated in the Microbiology Academy for Professional Development (MAPD) Workshop at ASM, as part of her winning a 2016 ASM Undergraduate Research Fellowship.
– Our two senior undergraduates, Kokila Shankar & Chris Sharkey, have finished their Bachelor’s degrees! Kokila will be heading to graduate school and Chris to computational data science at Microsoft. Congratulations on your honors thesis Kokila (first in the lab!) and your computational manuscript draft Chris!
- Moriah Szpara gave a talk at the 9th Workshop in Virus Evolution, on our collaboration with Nancy Sawtell (Cincinnati Children’s Hospital) & Rick Thompson (University of Cinncinnati), which also involves Utsav Pandey & Daniel Renner.
- Molly Rathbun joins the lab as our newest graduate student, from the BMMB graduate program. Molly has a background in forensics. Congratulations to Molly for being selected for the CBIOS Training Grant program!
- Utsav’s paper on the capture and comparison of herpesvirus genomes from dust and feather has been accepted at mSphere. Congratulations to Utsav, Daniel, Andy Bell, David Kennedy, and the team from the Read lab that contributed to this accomplishment!
– Colleen Mangold received a fellowship from the American Heart Association! She will be one of the Great Rivers Affiliate Winter 2016 Postdoctoral Fellows, with a two year award. Our colleagues Tony Huang, David Hughes, and Stephen Benkovic contributed to the process as co-sponsors. Congratulations Colleen!
– Chad Kuny joined the lab as a postdoc, bringing his expertise with beta-herpesviruses and more recently in RNA virology. Welcome Chad!
– Utsav gave a talk at the MCIBS and Pathobiology Summer Retreat, sharing his MDV genomics research with the new recruits.
- Moriah Szpara was an invited speaker at the FASEB meeting on “Virus Structure and Assembly” from July 24‐29, in Colorado. Kudos to our colleague Rebecca Craven for her part in co-organizing this meeting.
– Moriah Szpara presented Utsav’s research on MDV genome surveillance in the field, at the 11th International Symposium on Marek’s Disease and Avian Herpesviruses in France.
- Mackenzie Shipley presented a short talk on her new research on herpesvirus infection of human neurons at the University of Pennsylvania symposium entitled Herpesviruses: Pathogenesis and Cancer on June 24. This year’s symposium honored the career of our colleague Dr. Jim Alwine.
- Moriah Szpara chaired the 35th Annual Summer Symposium in Molecular Biology at the University Park campus, on the topic of Living with our Viromes. Kudos to all the lab members who chaired sessions, presented posters, hosted speakers and attendees, and helped make this a great event! The symposium took place May 24-25, 2016.
– Congratulations to our undergraduate researcher Apoorva Talanki, on her recent graduation from Penn State!
- Congratulations to Kokila Shankar, on winning a 2016 ASM Undergraduate Research Fellowship! This will support her summer research project, and attendance of the ASM Microbe meeting next year.
– The lab enjoyed a great day of cross-campus virology at the 2nd Penn State Intercampus Virology (PSIV) meeting. Over 60 people from labs spanning the University Park and Hershey College of Medicine campuses gathered for a day of science, networking, and talks by trainees. Colleen Mangold launched the day with her talk on acoustofluidics.
- Utsav Pandey reprised his MDV talk at the 2015 Northeastern Conference on Avian Diseases (NECAD).
- Mackenzie Shipley successfully completed her comprehensive exam as a BMMB graduate student! Congratulations!
– Utsav Pandey, Mackenzie Shipley, Daniel Renner, & Moriah Szpara present new data at the International Herpesvirus Workshop (IHW) in Boise, ID. In addition to our four posters, Mackenzie’s abstract was selected for a travel award, and Utsav gave a talk in the Veterinary Herpesvirus Satellite Workshop.
– Utsav Pandey successfully completed his comprehensive exam as a BMMB graduate student! Congratulations!
- Utsav Pandey and Mackenzie Shipley give talks at the inaugural PSU Intercampus Virology meeting, at the Penn State Hershey campus. Nicely done! The meeting on June 9 was organized by our colleagues John Wills & Becky Craven. The next one will be in November 2016 at the University Park campus.
– Utsav Pandey gives his first public research talk at a University of Pennsylvania symposium entitled Herpesviruses: Pathogenesis and Cancer on June 2. This year’s symposium honored the career of our colleague Dr. Nigel Fraser.
– Welcome to our newest undergraduate members of the lab: Kokila Shankar & Apoorva Talanki!
– Congrats to our undergrads Nathan Arnett & Chris Sharkey on landing great summer interships. Nathan will be at the University of Pittsburgh Summer Undergraduate Research Program (SURP), and Chris has an internship at Humana.
- Moriah Szpara gave a talk at the 8th Workshop in Virus Evolution.
– Our latest research is published in mBio — a lovely story about sister clones of various HSV strains. The early stages of this project involved Lance Parsons and Yoly Tafuri, in Lynn Enquist’s lab at Princeton. The research was brought to fruition with the help of several members of the new team at PSU — Jacob Shreve, Chris Bowen & Mackenzie Shipley. Nice teamwork!
- Daniel Renner joins the lab as a computational scientist & bioinformatics specialist. Welcome Daniel!
– Nathan Arnett wins a 2014-2015 ECoS Undergraduate Research Support Award.
- Yinan Wan, our collaborator in Istvan Albert’s group, completes the oral defense of her master’s thesis.
- Colleen Mangold joins the lab as a postdoctoral researcher, brining her expertise in neurobiology, aging, and pharmacology. Welcome Colleen!
- Moriah & Chris Bowen present a poster at the 39th Annual International Herpesvirus Workshop (IHW) in Japan.
– Jacob Shreve becomes our first lab alum, as he leaves to attend medical school at Indiana University School of Medicine.
- Moriah and other health researchers at Penn State build bridges with Penn State’s new visualization wall.
– PSU News carries Huck Institutes Seth Palmer’s article about our recent publication in the Journal of Virology.
- Chuck Grose comments in Journal of Virology about our recent paper. He begins, “An unintended consequence of phylogenetics is that it provides answers to questions that investigators were unaware they needed to ask.…”
– Latest publication: “Evolution and Diversity in Human Herpes Simplex Virus Genomes” goes online at Journal of Virology.
– Jacob Shreve joins the lab as Computational Scientist and bioinformatics specialist. Welcome Jacob!
– Huck Institutes writer Seth Palmer profiles Szpara Lab research.
– Chris Bowen joins the lab as Research Technologist and lab manager. Welcome Chris!
- Beginning of the Szpara Lab at PSU
New neuron-like cells (January 2018)
|Father-son transmission of HSV-1 (October 2017)|
Priscilla Schaffer Award (July 2017)
|Viruses can reveal your travel history (April 2016)
Have Herpes, Will Travel (April 2014)
Quantifying herpesvirus variation (February 2014)
New visualization wall in CyberHealth (February 2014)
BMB Welcomes Dr. Moriah Szpara (March 2013)